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ßá^Ÿâÿ G © · ã *4KÑ7C9)G¶Á+‰“W¡ÂSóü Ï b|:px‹@ƒÝ`|’ß(;w¶Þèf‰A € ¿ PA`Á…K«Æaÿ	�y’ " Gãÿ$&æ(—ÊôBœ\Œ˜ö1p¯CŠ - I È Û TI<È[åG~–™þ>ƒ	ñ	î>»Ü£(y=L   ” µMlÝ‡ž)ó1¼;+7m(œ¥3ô_ï" ,w”ÝŒJfé×9Ö	zQ}�#×ÄÁL @ @ª´ÎG§ó�r
DÒBŒÈä<™ý^¨(Ô' ‘žF��±5Ó	\	s		
-
±.Â•Yô%k£µ]õø P ¡ ¹ Ä Ö &8Sºrý2×…çü		D	P	
¸éaC ßë90b	:¯~õ+ÜÐ Þ æ \�òY �öù“	ìIX£ÃÉ$U¹ ¬ 3áFÎ6BEjp†	Ö
ÎFy…lrÃ*vxòûþ  FO{@_ÇZwäÜý'þ	'µçío®‘ˆK “Ü‹Ü?†è»Ö6-A‹Ç˜]§ó"^î O   « Ã å R¹8ø„a	WxkqÈ®}*`# L M N ž Ÿ CDE?’Æ67YÛÛƒý	5´­\¦)  ,2  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                            $                                                                                                                                                     	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            	                                                                                                                         	         #         
                                                                                                                                                                           
      	         
               ,            	                                                         
                              
                     &                           k   Q      !               Q                                                                                                                                                                       $                                                                     	                                                                                                                                                                                                                                                                          	                                             	   #   
                                                           
  	   
     ,    	                   
          
       &         k Q  !     Q   gN  hT  hn  h   l€  p   uðf c" " È ß@_`’µ89Yr’�'…(}`a"Lk²Ð^ñ9	›3æeæR `2yóàcì
áòòì
á2è0	/ k2þ¹ÄŒ’4ˆ`fµè0	ø-:v½Õ2Yyó2>DM]{P,Â<åN¯Íò
Íò
ånÍò
ò
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Éêk²Ðß–^ º	L	³}»_ý
aS%ò
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&†	âcuN z	4réŠãR¹v··ru
3‘KžÓcøæ
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‰cT
¹éÓüQYLrÁ‘KžÓc
  ©KÝ‡:SsÜzPp:çê90Õ	:[¸2+¬ê.ãØOêÜ&Pp.­ °wíè0	YÑ ®Ä	¤n&»î	Èý`Ý<Häô  E �½\	L	í	“­ãêò{yØG`êrK9)p÷j …›uPúP  RS¹º9ÎÛ0aj�	P	6m…(´Òçîä(y`fÔ^'6
™
Î a_Ý@œoŠ?:Ý{CÁüÜþ
†0ÿG	AÞ6ÀQÜþ
ÛÿÎ�Òä(Ô':OS[¸s2+¬êÜ&zPpÐ¼	  ©K9Î0�Òä(Ô'O!Ž	Ÿk 4 d7M…´oŠÀÄíñU0êèû0	C	»Â6Wì7ø	Y{04Î�Òîä(Ô'‰
  íè0	«©¸K‰YúP�ŒâîUÝÓÝãØ`ïÉf:
(šõçîõÆ
°Ö“ž¨½iÚÎRH Ú }‚	¢ÜPp`üêrKQ9)YL˜i<ŠÛýE	«éæ.	©c‰
øW:‰
çhŽ¹ø3…Á¤[„é oÑ3†Ð±öq�Õ(1mOˆ	¼	ô
2à�£÷”
¾ voŠTIv	î¡E`·k†ý<Hv	¡Eøj p…÷
›	5 ¿„Ô– Íip%Ï#s%co
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  ö<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4389-4398" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="1" name="3xMyc" directionality="1" translationMW="3573.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5639" maxFusedRunOn="5639" detectionMode="exactProteinMatch"><Segment range="4398-4487" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;3 tandem Myc epitope tags&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EQKLISEEDLEQKLISEEDLEQKLISEEDL"/></Q></Feature><Feature recentID="2" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5720-5768" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5640-5645" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="other DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="4" 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text="aph(3')-II (or nptII)"/></Q><Q name="note"><V text="NeoR/KanR;confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase from Tn5"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="10" name="SV40 ori" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2101-2236" color="#ffff00" type="standard"/><Q name="note"><V text="SV40 ori;SV40 origin of replication"/></Q></Feature><Feature recentID="11" name="Psv40" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" 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text="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"/></Q></Feature><Feature recentID="14" name="Pcmv" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3610-3813" color="#ffffff" type="standard"/><Q name="note"><V text="CMV promoter;human cytomegalovirus (CMV) immediate early promoter"/></Q></Feature><Feature recentID="15" name="CMV enhancer" type="enhancer" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3814-4117" color="#ffffff" type="standard"/><Q name="note"><V text="CMV enhancer;human cytomegalovirus immediate early enhancer"/></Q></Feature><Feature recentID="16" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" 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   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  -<Notes>
<UUID>2e005362-7d2c-4b9f-ac04-2fa9b9a60e94</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-3Ã—Myc-KCMF1(human)-CMV-MCS-EGFP-Neo</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.6.16</Created>
<LastModified UTC="20:41:24">2025.7.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference type="Journal Article" authors="admin" journal="Exported 2015-10-18&amp;nbsp;&amp;nbsp; from MLCC &lt;a href='http://www.miaolingbio.com'>http://www.miaolingbio.com&lt;/a>" journalName="Exported 2015-10-18&amp;nbsp;&amp;nbsp; from MLCC &lt;a href='http://www.miaolingbio.com'>http://www.miaolingbio.com&lt;/a>" title="Direct Submission"/>
</References>
</Notes>
  Y     K         
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